• Chemical Diversity as a Function of Temperature in Six Northern Diatom Species 

      Huseby, Siv; Degerlund, Maria; Eriksen, Gunilla Kristina; Ingebrigtsen, Richard Andre; Eilertsen, Hans Christian; Hansen, Espen (Journal article; Tidsskriftartikkel; Peer reviewed, 2013)
      In this study, we investigate how metabolic fingerprints are related to temperature. Six common northern temperate diatoms (Attheya longicornis, Chaetoceros socialis, Chaetoceros furcellatus, Porosira glacialis, Skeletonema marinoi, and Thalassiosira gravida) were cultivated at two different temperatures, 0.5 and 8.5 °C. To exclude metabolic variations due to differences in growth rates, the growth ...
    • The Effect of Freeze/Thaw Cycles on Reproducibility of Metabolic Profiling of Marine Microalgal Extracts Using Direct Infusion High-Resolution Mass Spectrometry (HR-MS) 

      Eilertsen, Hans Christian; Huseby, Siv; Degerlund, Maria; Eriksen, Gunilla Kristina; Ingebrigtsen, Richard Andre; Hansen, Espen (Journal article; Tidsskriftartikkel; Peer reviewed, 2014-10-13)
      During normal sample preparation, storage in freezers and subsequent freeze/thaw cycles are commonly introduced. The effect of freeze/thaw cycles on the metabolic profiling of microalgal extracts using HR-MS was investigated. Methanolic extracts of monocultures of Arctic marine diatoms were analyzed immediately after extraction, after seven days of storage at −78 °C (one freeze/thaw cycle), and after ...
    • Structural and functional analyses of Rubisco from arctic diatom species reveal unusual posttranslational modifications 

      Valegård, Karin; Andralojc, P. John; Haslam, Richard P.; Pearce, F. Grant; Eriksen, Gunilla Kristina; Madgwick, Pippa J.; Kristoffersen, Anne Karin; van Lun, Michiel; Klein, Uwe; Eilertsen, Hans Christian; Parry, Martin A.J.; Andersson, Inger (Journal article; Tidsskriftartikkel; Peer reviewed, 2018-06-20)
      The catalytic performance of the major CO<sub>2</sub>-assimilating enzyme, ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), restricts photosynthetic productivity. Natural diversity in the catalytic properties of Rubisco indicates possibilities for improvement. Oceanic phytoplankton contain some of the most efficient Rubisco enzymes, and diatoms in particular are responsible for a significant ...